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KMID : 1098420140220040276
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Korean Journal of Medicinal Crop Science
2014 Volume.22 No. 4 p.276 ~ p.288
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Isolation of Polysaccharides Modulating Intestinal Immune System and Single Oral Dose Toxicity Test in Astragalus membranaceus Abovegroud Parts
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Choi Li-Na
Park Yeong-Chul
Lee Ji-Sun
Kim Jung-Woo
Kim Jong-Bong
Cheoi Yu-Soon
Kim Kwang-Ki
Lee Jae-Keun
Yu Chang-Yeon
Kim Seung-Hyun
Chung Ill-Min
Kim Jae-Kwang
Lim Jung-Dae
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Abstract
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The six polysaccharide fractions were prepared by chromatographic procedure from the hot water extracts of the aboveground parts of Astragalus membranaceus. These six polysaccharides from aboveground parts of Astragalus membranaceus Bunge were tested for gut-mucosal immune activity and acute toxicity. In a view of molecular weight, the six fractions were estimated to be 75000, 88000, 129000 and 345000 Da, respectively. Component sugar analysis indicated that these fractions were mainly consisted of galactose (46.3 ~ 11.8%) and arabinose (35.4 ~ 9.9%) in addition to glucose, rhamnose, fucose, arabinose, xylose, mannose, glucuronic acid and galacturonic acid. Among the six major purified polysaccharides, AMA-1-b-PS2 showed highest bone merrow cell proliferation and lymphocyte of Peyer’s patch stimulating activity. It may be concluded that intestinal immune system modulating activity of aboveground parts from Astragalus membranaceus Bunge is caused by polysaccharides having a polygalacturonan moiety with neutral sugars such as arabinose and galactose. In single oral dose toxicity study, no differences were observed between control and treated groups in clinical signs. The results indicated that lethal dose 50 (LD50) of water extracts from Astragalus membranaceus-aboveground parts was found to be higher than 5000 ㎎/㎏/day in this experiment. From the above results, we may suggest that Astragalus membranaceus-aboveground parts might have useful as a safe material for functional food and pharmaceutics.
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KEYWORD
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Astragalus membranaceus, Aboveground Parts, Polysaccharide, Intestinal Immune System Modulating Activity, Single Dose Oral Toxicity
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